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:: Volume 5, Issue 2 (2019) ::
Sustainable Aquaculture. Health. Management. J. 2019, 5(2): 54-66 Back to browse issues page
Inhibitory activity of native probiotic Bacillus vallismortis IS03 against pathogenic Vibrio harveyi under in vitro and in vivo conditions in Litopenaeus vannamei
M Mahjoub , M Mirbakhsh * , M Afsharnasab , S Kakoolaki , S Hosseinzadeh
Abstract:   (4020 Views)
An excellent alternative for chemical antimicrobial agents to prevent disease in the shrimp aquaculture is the application of effective probiotics. The present study was evaluated the effect of Bacillus vallismortis IS03 as a native probiotic, isolated from digestive tract of Litopenaeus vannamei on pathogenic Vibrio harveyi under in vitro and in vivo circumstances. Co-cultivation of V. harveyi and B. vallismortis showed significantly (P<0.05) decreased the growth of V. harveyi in the treatment groups compared to the control. Cell-free extracts of B. vallismortis IS03 exhibited more appropriate antibacterial effects on replication of V. harveyi. The highest and lowest inhibitory effects were respectively shown in108 and 106 CFU ml-1 of B. vallismortis IS03 cell-free extracts. The probiotic potential of B. vallismortis IS03 was assessed through the groups of control and the experiments 106, 107 and 108 CFU ml-1 salt water once every 3 days from zoea1 process to end point of the study. The probiotic potential of B. vallismortis IS03 was assessed through the groups of control and the experiments 106, 107 and 108 CFU ml-1 salt water once every 3 days from zoea1 process to end point of the study. Shrimp survival was determined after 10 days of challenge with V. harveyi  at 10 5 CFU ml-1 (for the first 5 days) and 10 7 CFU ml-1  (for the second 5 days).The cumulative mortality in the treatment with 108 CFU ml-1 of  B. vallismortis IS03 reached 11.88% compared to 40.63% in the control group. At the end of the trial, total bacterial counts on TSA, total vibrio on TCBS were significantly (P<0.05) lower in the 108 CFU ml-1 treatment group. Bacillus counts on MYP agar in the treatment groups were significantly (P<0.05) higher than the control, also total bacterial counts was lower in the treatment groups, while, no vibrio were grown in the muscle tissues of shrimp treated with probiotics. It is concluded that 108 CFU ml-1 of probiotic, B. vallismortis IS03 has antibacterial efficiency against pathogenic V. harveyi at in vitro and in vivo conditions.
Keywords: Probiotic, Bacillus vallismortis, Litopenaeus vannamei, Vibrio harveyi
Full-Text [PDF 510 kb]   (954 Downloads)    
Type of Study: Original research papers | Subject: Bacterial Disease
Received: 2018/11/22 | Accepted: 2019/07/24 | Published: 2019/11/20
References
1. Ajitha S., Sridhar M., Sridhar N., Singh I.S.B. & Varghese V. (2004) Probiotic effects of lactic acid bacteria against Vibrio alginolyticuys in Penaeus (Fenneropenaeus) indicus (H. Milne Edwards). Asian Fisheries Science 17, 71-80.
2. Alavandi S.V., Vijayan K.K., Santiago T.C., Poornima M., Jithendran K.P., Ali S.A. & Rajan J.J.S. (2004) Evaluation of Pseudomonas spp. PM 11 and Vibrio fluvialis PM 17 on immune indices of tiger shrimp, Penaeus monodon. Fish & Shellfish Immunology 17, 115-120. [DOI:10.1016/j.fsi.2003.11.007] [PMID]
3. Austin B., Stuckey L.F., Robertson P.A.W., Effendi I., Griffith D.R.W. (1995) A probiotic strain of Vibrio alginolyticus effective in reducing diseases caused by Aeromonas salmonicida, Vibrio anguillarum and Vibrio ordalii. Journal of Fish Diseases 18(1), 93-96. [DOI:10.1111/j.1365-2761.1995.tb01271.x]
4. Balcazar J.L. & Rojas-Luna T. (2007) Inhibitory activity of probiotic Bacillus subtilis UTM126 against Vibrio species confers protection against vibriosis in juvenile shrimp (Litopenaeus vannamei). Current microbiology 55, 409-412. [DOI:10.1007/s00284-007-9000-0] [PMID]
5. Baruzzi F., Quintieri L., Morea M. & Caputo L. (2011) Antimicrobial compounds produced by Bacillus spp. And applications in food. Science against microbial pathogens: Communicating Current Res Technological Advances A. Mendez- Vilas (Ed.), 1102-1111.
6. Chaurasia B., Pandey A., Palni L.M.S., Trivedi P., Kumar B. & Colvin N. (2005) Diffusible and volatile compounds produced by an antagonistic Bacillus subtilis strain cause structural deformations in pathogenic fungi in vitro. Microbiological research 160, 75-81. [DOI:10.1016/j.micres.2004.09.013] [PMID]
7. Chio C-H., Guu Y-K., Pan T-M. & Cheng W., (2007) Immune responses and gene expression in white shrimp; Litopenaeus vanammei, induced by Lactobacillus plantarum. Fish Shellfish Immune 31, 196-201.
8. Chun J., Lee J-H., Jung Y., Kim S., Kim B.K. & Lim Y.W. (2007) Ez Taxomn: a web-based tool for the identification of prokaryotes based on 16S ribosomal RNA gene sequences. International journal of systematic and evolutionary microbiology 57, 2259- 2261. [DOI:10.1099/ijs.0.64915-0] [PMID]
9. Chytjanya R., Karunasagar L. & Karunasagar I. (2002) Inhibition of shrimp pathogenic vibriosis by a marine Pseudomonas 1-2 strain. Aquaculture 208 (1-2), 1-10. [DOI:10.1016/S0044-8486(01)00714-1]
10. Dalmin G., Kathiresan K. & Purushothaman A., (2001) Effect of probiotic on bacterial population and health status of shrimp in culture pond ecosystem. Indian J Exp Biol, 39(9), 939 -942.
11. Drablos F., Nicholson D. & Ronning M. (1999) EXAFS study of zinc coordination in Bacitracin A. Biochimica et Biophysica Acta (BBA)-Protein Structure and Molecular Enzymology 1431, 433-442. [DOI:10.1016/S0167-4838(99)00064-3]
12. Farzanfar A. (2006) The use of probiotics in shrimp aquaculture. FEMS Immunology & Medical Microbiology 48(2), 149-158. [DOI:10.1111/j.1574-695X.2006.00116.x] [PMID]
13. Gatesoupe F.J. (1999) The use of probiotics in aquaculture. Aquaculture 180, 147- 165. [DOI:10.1016/S0044-8486(99)00187-8]
14. Gomez-Gil B., Roque A. & Velasco-Blanco G. (2002) Culture of Vibrio alginolyticus C7b, a potential probiotic bacterium, with the microalga Chaetoceros muelleri, Aquaculture 211(1-4), 43-48. [DOI:10.1016/S0044-8486(02)00004-2]
15. Green D.H., Wakeley P.R., Page A., Barnes A., Baccigalupi L., Ricca E. & Cutting S.M. (1999) Characterization of two Bacillusprobiotics. Appl. Environ. Microbiol 65(9), 4288-4291. [DOI:10.1128/AEM.65.9.4288-4291.1999]
16. Gullian M., Thompson F. & Rodriguez J. (2004) Selection of probiotic bacteria and study of their immunostimulatory effect in Penaeus vannamei. Aquaculture 233, 1-14. [DOI:10.1016/j.aquaculture.2003.09.013]
17. Holt J.G., Krieg N.R., Sneath P.H.A., Staley J.T. & Williams S.T. (1994) Bergey's Manual of Determinative Bacteriology, 9th ed. Williams and Wilkins, Baltimore, pp. 260-274.
18. Immanuel G., Citarasu T., Sivaram V. & Palavesam, A. (2007) Delivery of HUFA probionts and biomedicine through bioencapsulated Artemia as a means to enhance the growth and survival and reduce the pathogenesity in shrimp penaeus monodon postlarvae. Aquaculture International 15, 137-152. [DOI:10.1007/s10499-007-9074-5]
19. Karunasagar Y., Pai R. & Malaty G.R. (1994) Mass mortality of Penaeus monodon larvae due to antibiotic resistant Vibrio harveyi infection. Aquaculture 128, 203-209. [DOI:10.1016/0044-8486(94)90309-3]
20. Kongnum K. & Hongpattarakere T. (2012) Effect of Lactobacillus plantarum isolated from digestive tract of wild shrimp on growth and survival of white shrimp (Litopenaeus vannamei) challenged with Vibrio harveyi. Fish & Shellfish Immunology 32, 170-177. [DOI:10.1016/j.fsi.2011.11.008] [PMID]
21. Lee C-S. (2003). Biotechnological advances in finish hatchery Production: a review. Aquaculture 227 (1-4), 439-458. [DOI:10.1016/S0044-8486(03)00522-2]
22. Liu K-F., Chiu C-H., Shiu Y-L., Cheng W. & Liu, C-H. (2010) Effects of the probiotic, Bacillus subtilis E20, on the survival, development, stress tolerance, and immune status of white shrimp, Litopenaeus vanammei larvae. Fish & Shellfish Immunology 28, 837-844. [DOI:10.1016/j.fsi.2010.01.012] [PMID]
23. Meunpol O., Lopinyosiri K. & Menasveta P. (2003) The effects of ozone and probiotics on the survival of black tiger shrimp (Penaeus monodon). Aquaculture 220, 437-448. [DOI:10.1016/S0044-8486(02)00586-0]
24. Mirbakhsh M., Akhavan sepahy A., Afsharnasab M., Khanafari A. & Razavi M.R. (2014) Molecular identification of Vibrioi harveyi from larval stage of pacific white shrimp (Litopenaeus vanammei) Boone (Crustacea; Decapoda) by polymerase chain reaction and 16S rDNA Sequancing, Iranian Journal of Fisheries Sciences 13(2), 384-393.
25. Mirbakhsh M., Akhavansepahy A., Afsharnasab M., Khanafari A. & Razavi M.R. (2013) Screening and evaluation of indigenous bacteria from the Persian Gulf as a probiotic and biocontrol agent against Vibrio harveyi in Litopenaeus vannamei post larvae. Iranian Journal of Fisheries Sciences 12(4), 873-886.
26. Moriarty D.J.W. (1999) Disease control in shrimp aquaculture with probiotic bacteria. Paper presented at the 8th Intertational Symposium on Microbial Ecology. Halifax, Canada.
27. Morikawa M., Ito M. & Imanaka T. (1992) Isolation of a new surf- actin producer Bacillus pumilus A-1, and cloning and nucleotide sequence of the regulator gene, psf-1. Journal of fermentation and bioengineering 74, 255-261. [DOI:10.1016/0922-338X(92)90055-Y]
28. Perez C., Suarez C. & Castro GR. (1993) Antimicrobial activity determined in strains of Bacillus circulans cluster. Folia microbiologica 38, 25-28. [DOI:10.1007/BF02814544] [PMID]
29. Rengpipat S., Phianphak W., Piyatiratitivorakul S. & Menasveta P. (1998a) Effects of a probiotic bacterium on black tiger shrimp Penaeus monodon survival and growth. Aquaculture 167, 301-313. [DOI:10.1016/S0044-8486(98)00305-6]
30. Rengpipat S., Rukpratanporn S., Piyatiratitvorakul S. & Menasaveta P. (2000) Immunity enhancement in black tiger shrimp (penaeus monodon ) by a probiont bacterium (Bacillus S11) . Aquaculture 191 (4), 271-288. [DOI:10.1016/S0044-8486(00)00440-3]
31. Swain S.M. & Singh C. (2009) Inhibitory activity of probiotic Streptococcus phocae PI80 and Enterococcus faecium MC13 against Vibriosis in shrimp. World Journal of Microbiology and Biotechnology 25, 697- 703. [DOI:10.1007/s11274-008-9939-4]
32. Tseng D-Y., Ho P-L., Huang S-Y., Cheng S-C., Shiu Y-L., Chio C-H. & Lio C-H. (2009) Enhancement of immunity and disease resistance in the white shrimp, Litopenaeus vanammei, by the probiotic, Bacillus subtilis E20. Fish Shellfish Immunology 26, 339-344. [DOI:10.1016/j.fsi.2008.12.003] [PMID]
33. Vaseeharan B. & Ramasamy P. (2003) Control of pathogenic Vibrio spp. by Bacilus subtilis BT23, a possible probiotic treatment for black tiger shrimp penaeus monodom. Letters in applied microbiology 36(2), 83-87. [DOI:10.1046/j.1472-765X.2003.01255.x] [PMID]
34. Verschure LRG, Sorgeloos P. & Verstreate W. (2000) Probiotic bacteria as biological control agents in aquaculture. Microbiology and molecular Biology Reviews 64, 655-671. [DOI:10.1128/MMBR.64.4.655-671.2000] [PMID] [PMCID]
35. Wang Y-B. (2007) Effect of probiotics on growth performance and digestive enzyme activity of the shrimp Penaeus vanammei. Aquaculture 269, 259-294. [DOI:10.1016/j.aquaculture.2007.05.035]
36. Yilmaz M., Soran H. & Beyatli Y. (2006) Antimicrobial activities of some Bacillus spp. strains isolated from the soil. Microbiological research 161, 127-131. [DOI:10.1016/j.micres.2005.07.001] [PMID]
37. Ziaei-Nejad S., Habibi Rezaei M., Azari Takami Gh., Lovett L.D., Mirvaghefi A.R. & Shakouri M. (2006) The effect of Bacillus spp. bacteria used as probiotics on digestive enzyme activity, survival and growth in the Indian white shrimp Fenneropenaeus indicus. Aquaculture 252, 516-524. [DOI:10.1016/j.aquaculture.2005.07.021]
38. Zokaeifar H., Saad C.R.B, Doud H.M., Harmin S. A. & Shakibzadeh S. (2009) Effect of Bacillus subtilis on the growth and survival rate of shrimp (Litopenaeus vanname). African Journal of Biotechnology 8 (14), 3369-3376.

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Mahjoub M, Mirbakhsh M, Afsharnasab M, Kakoolaki S, Hosseinzadeh S. Inhibitory activity of native probiotic Bacillus vallismortis IS03 against pathogenic Vibrio harveyi under in vitro and in vivo conditions in Litopenaeus vannamei. Sustainable Aquaculture. Health. Management. J. 2019; 5 (2) :54-66
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