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Reproductive Biomedicine
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Volume 15, Issue 1 (1-2017)
IJRM 2017, 15(1): 33-40 |
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Dorostghoal M, Ghaffari H, Shahbazian N, Mirani M. Endometrial expression of β3 integrin, calcitonin and plexin-B1 in the window of implantation in women with unexplained infertility. IJRM 2017; 15 (1) :33-40
URL: http://ijrm.ir/article-1-790-en.html
URL: http://ijrm.ir/article-1-790-en.html
1- Department of Biology, Faculty of Sciences, Shahid Chamran University of Ahvaz, Ahvaz, Iran. , dorostghoal@gmail.com
2- Hematology, Oncology and Stem Cell Transplantation Research Center, Tehran University of Medical Sciences, Tehran, Iran.
3- Department of Obstetrics and Gynecology, Imam Khomeini Hospital, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
4- Department of Biology, Faculty of Sciences, Shahid Chamran University of Ahvaz, Ahvaz, Iran.
2- Hematology, Oncology and Stem Cell Transplantation Research Center, Tehran University of Medical Sciences, Tehran, Iran.
3- Department of Obstetrics and Gynecology, Imam Khomeini Hospital, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
4- Department of Biology, Faculty of Sciences, Shahid Chamran University of Ahvaz, Ahvaz, Iran.
Abstract: (2695 Views)
Background: Endometrial receptivity plays a key role in the establishment of successful implantation and its impairment may contribute to subfertility and limit the assisted reproduction techniques (ART) success.
Objective: The aim of present study was to investigate endometrial receptivity in terms of β3 integrin, calcitonin and plexin-B1 expression in women with unexplained infertility.
Materials and Methods: We evaluated expression of β3 integrin, calcitonin and plexin-B1 through mRNA level measurement with real-time RT-PCR, in the endometrium of 16 infertile patients with unexplained infertility and 10 fertile women. Endometrial biopsies were collected during a single menstrual cycle on postovulatory day LH+7 in each subject.
Results: Significant differences regarding β3 integrin and calcitonin expression levels found between patients with unexplained infertility and the fertile women. Endometrial plexin-B1 expression levels showed no significant difference between fertile and infertile women. There were significant correlations between expression of β3 integrin with calcitonin and plexin-B1 in fertile and infertile women.
Conclusion: Reduced in endometrial expression of β3 integrin and calcitonin alone or together may contribute to unexplained infertility and these genes could account as the potential molecular markers of infertility
Objective: The aim of present study was to investigate endometrial receptivity in terms of β3 integrin, calcitonin and plexin-B1 expression in women with unexplained infertility.
Materials and Methods: We evaluated expression of β3 integrin, calcitonin and plexin-B1 through mRNA level measurement with real-time RT-PCR, in the endometrium of 16 infertile patients with unexplained infertility and 10 fertile women. Endometrial biopsies were collected during a single menstrual cycle on postovulatory day LH+7 in each subject.
Results: Significant differences regarding β3 integrin and calcitonin expression levels found between patients with unexplained infertility and the fertile women. Endometrial plexin-B1 expression levels showed no significant difference between fertile and infertile women. There were significant correlations between expression of β3 integrin with calcitonin and plexin-B1 in fertile and infertile women.
Conclusion: Reduced in endometrial expression of β3 integrin and calcitonin alone or together may contribute to unexplained infertility and these genes could account as the potential molecular markers of infertility
Type of Study: Original Article |
References
1. 90TAghajanova L, Hamilton AE, Giudice LC. 45T90TUterine receptivity to human embryonic implantation45T90T: 45T90Thistology45T90T, 45T90Tbiomarkers, and transcriptomics45T90T. Semin Cell Dev Biol 2008; 19: 204-211. [DOI:10.1016/j.semcdb.2007.10.008]
2. Achache H, Revel A. Endometrial receptivity markers, the journey to successful embryo implantation. Hum Reprod Update 2006; 12: 731-746. [DOI:10.1093/humupd/dml004]
3. Strowitzki T, Germeyer A, Popovici R, von Wolff M. The human endometrium as a fertility-determining factor. Hum Reprod Update 2006; 12: 617-630. [DOI:10.1093/humupd/dml033]
4. 65THaouzi D65T, 65TMahmoud K65T, 65TFourar M65T, 65TBendhaou K65T, 65TDechaud H65T, 65TDe Vos J65T, et al. Identification of new biomarkers of human endometrial receptivity in the natural cycle. Hum Reprod 2009; 24: 198-205.
5. Cakmak H, Taylor HS. Implantation failure: molecular mechanisms and clinical treatment. Hum Reprod Update 2011; 17: 242-253. [DOI:10.1093/humupd/dmq037]
6. Sharkey AM, Smith SK. The endometrium as a cause of implantation failure. Best Pract Res Clin Obstet Gynaecol 2003; 17: 289-307. [DOI:10.1016/S1521-6934(02)00130-X]
7. Illera MJ, Lorenzo PL, Gui YT, Beyler SA, Apparao KB, Lessey BA. A role for alphavbeta3 integrin during implantation in the rabbit model. Biol Repod 2003; 68: 766-771. [DOI:10.1093/biolreprod/68.3.766]
8. Tei C, Maruyama T, Kuji N, Miyazaki T, Mikami M, Yoshimura Y. Reduced expression of alphavbeta3 integrin in the endometrium of unexplained infertility patients with recurrent IVF-ET failures: improvement by danazol treatment. J Assist Reprod Genet 2003; 20: 13-20. [DOI:10.1023/A:1021254620888]
9. Illera MJ, Cullinan E, Gui Y, Yuan L, Beyler SA, Lessey BA. Blockade of the αvβ3 integrin integrin adversely affects implantation in the mouse. Biol Reprod 2000; 62: 1285-1290. [DOI:10.1095/biolreprod62.5.1285]
10. Lessey BA, Castelbaum AJ, Sawin SW, Sun J. Integrins as markers of uterine receptivity in women with primary unexplained infertility. Fertil Steril 1995; 63: 535-542. [DOI:10.1016/S0015-0282(16)57422-6]
11. 65TMeyer WR65T, 65TCastelbaum AJ65T, 65TSomkuti S65T, 65TSagoskin AW65T, 65TDoyle M65T, 65THarris JE65T, et al. Hydrosalpinges adversely affect markers of endometrial receptivity. Hum Reprod 1997; 12: 1393-1398. [DOI:10.1093/humrep/12.7.1393]
12. Zhu LJ, Cullinan-Bove K, Polihronis M, Bagchi MK, Bagchi IC. Calcitonin is a progesterone-regulated marker that forecasts the receptive state of endometrium during implantation. Endocrinology 1998; 139: 3923-3934. [DOI:10.1210/endo.139.9.6178]
13. 65TXiong T65T, 65TZhao Y65T, 65THu D65T, 65TMeng J65T, 65TWang R65T, 65TYang X65T, et al. Administration of calcitonin promotes blastocyst implantation in mice by up-regulating integrin b3 expression in endometrial epithelial cells. Hum Reprod 2012; 12: 3540-3551.
14. Li HY, Shen JT, Chang SP, Hsu WL, Sung YJ. Calcitonin promotes outgrowth of trophoblast cells on endometrial epithelial cells: involvement of calcium mobilization and protein kinase C activation. Placenta 2008; 29: 20-29. [DOI:10.1016/j.placenta.2007.09.012]
15. Li Q, Wang J, Armant DR, Bagchi MK, Bagchi IC. Calcitonin down-regulates E-cadherin expression in rodent uterine epithelium during implantation. J Biol Chem 2002; 277: 46447-46455. [DOI:10.1074/jbc.M203555200]
16. Trusolino L, Comoglio PM. Scatter-factor and semaphoring receptors: cell signalling for invasive growth. Nat Rev Cancer 2002; 4: 289-300. [DOI:10.1038/nrc779]
17. Harduf H, Goldman S, Shalev E. Human uterine epithelial RL95-2 and HEC-1A cell-line adhesiveness: the role of plexin B1. Fertil Steril 2007; 87: 1419-1427. [DOI:10.1016/j.fertnstert.2006.11.141]
18. 90TAmir M, Romano S, Goldman S, Shalev E. 45T90TPlexin45T90T-45T90TB145T90T, 45T90Tglycodelin and MMP7 expression in the human fallopian tube and in the endometrium45T90T. Reprod Biol90T 2009; 7: 152.
19. 65TLi J65T, 65TLiang X65T, 65TChen Z65T. Improving the 57Tembryo implantation57T via novel molecular targets. 65TCurr Drug Targets65T 2013; 8: 864-871.
20. 65TCreus M65T, 65TOrdi J65T, 65TFábregues F65T, 65TCasamitjana R65T, 65TFerrer B65T, 65TColl E65T, et al. Integrin expression in normal and out-of-phase endometria. Hum Reprod 1998; 13: 3460-3468. [DOI:10.1093/humrep/13.12.3460]
21. Hii LLP, Roger PAW. Endometrial vascular and glandular expression of integrin αvβ3 integrin in women with and without endometriosis. Hum Reprod 1998; 13:1030-1035. [DOI:10.1093/humrep/13.4.1030]
22. Gonzalez RR, Palomino A, Boric A, Vega M, Devoto L. A quantitative evaluation of β1, β4, βV and β3 endometrial integrins of fertile and unexplained infertile women during the menstrual cycle. A flow cytometric appraisal. Hum Reprod 1999; 14: 2485-2492. [DOI:10.1093/humrep/14.10.2485]
23. 65THodivala-Dilke65T KM, 65TMcHugh65T KP, 65TTsakiris65T DA, 65TRayburn65T H, 65TCrowley65T D, 65TUllman-Culleré65T M, et al. β3-integrin-deficienct mice are a model for Glanzmann thrombasthenia showing placental defects and reduced survival. J Clin Invest 1999; 103: 229-238. [DOI:10.1172/JCI5487]
24. 65TCeydeli65T N,65T Kaleli65T S, 65TCalay65T Z, 65TErel65T CT, 65TAkbas65T F, 65TErtungealp65T E. Difference in αvβ3 integrin expression in endometrial stromal cell in subgroups of women with unexplained infertility. 90TEur J Obstet Gynecol Reprod Biol90T 2006; 126: 206-211. [DOI:10.1016/j.ejogrb.2005.11.034]
25. Amjadi F, Aflatoonian R, Javanmard SH, Saifi B, Ashrafi M, Mehdizadeh M. Apolipoprotein A1 as a Novel anti-implantation biomarker in polycystic ovary syndrome: A case-control study. J Res Med Sci 2015; 20:1039-1045. [DOI:10.4103/1735-1995.172813]
26. DuQuesnay R, Wright C, Aziz AA, Stamp GW, Trew GH, Margara RA, et al. Infertile women with isolated polycystic ovaries are deficient in endometrial expression of osteopontin but not alphavbeta3 integrin during the implantation window. Fertil Steril 2009; 91: 489-499. [DOI:10.1016/j.fertnstert.2007.11.063]
27. WHO. Laboratory Manual for the Examination of Human Semen and Sperm-cervical Mucus Interaction. 5th Ed. Cambridge, Cambridge University Press; 2010.
28. Noyes RW, Hertig AT, Rock J. Dating the endometrial biopsy. Am J Obstet Gynecol 1975; 122: 262-263. [DOI:10.1016/S0002-9378(16)33500-1]
29. Ye J, Coulouris G, Zaretskaya I, Cutcutache I, Rozen S, Madden TL. Primer-BLAST: A tool to design target-specific primers for polymerase chain reaction. BMC Bioinformatics 2012; 13: 134. [DOI:10.1186/1471-2105-13-134]
30. 30. Livak KJ, Schmittgen TD. Analysis of relative gene expression data using real-time quantitative PCR and the 2-DDCT method. Methods 2001; 25: 402-408. [DOI:10.1006/meth.2001.1262]
31. Revel A. Defective endometrial receptivity. Fertil Steril 2012; 97: 1028-1032. [DOI:10.1016/j.fertnstert.2012.03.039]
32. Macklon NS, Stouffer RL, Giudice LC, Fauser BC. The science behind 25 years of ovarian stimulation for in vitro fertilization. Endocr Rev 2006; 27: 170-207. [DOI:10.1210/er.2005-0015]
33. Tuckerman EM, Laird SM, Prakash A, Li TC. Expression of integrins in the endometrium of women with recurrent miscarriage. Fertil Steril 2006; 86: 755-757. [DOI:10.1016/j.fertnstert.2006.02.099]
34. Xu B, Sun X, Li L, Wu L, Zhang A, Feng Y. Pinopodes, leukemia inhibitory factor, integrin-beta3, and mucin-1 expression in the peri-implantation endometrium of women with unexplained recurrent pregnancy loss. Fertil Steril 2012; 98: 389-395. [DOI:10.1016/j.fertnstert.2012.04.032]
35. Coughlan C, Sinagra M, Ledger W, Li TC, Laird S. Endometrial integrin expression in women with recurrent implantation failure after in vitro fertilization and its relationship to pregnancy outcome. Fertil Steril 2013; 100: 825-830. [DOI:10.1016/j.fertnstert.2013.05.007]
36. Othman R, Omar MH, Shan LP, Shafiee MN, Jamal R, Mokhtar NM. Microarray profiling of secretory-phase endometrium from patients with recurrent miscarriage. Reprod Biol 2012; 12: 183-199. [DOI:10.1016/S1642-431X(12)60085-0]
37. Germeyer A, Savaris RF, Jauckus J, Lessey B. Endometrial beta3 integrin profile reflects endometrial receptivity defects in women with unexplained recurrent pregnancy loss. Reprod Biol Endocrinol 2014; 12: 53. [DOI:10.1186/1477-7827-12-53]
38. Franasiak JM, Holoch KJ, Yuan L, Schammel DP, Young SL, Lessey BA. Prospective assessment of midsecretory endometrial leukemia inhibitor factor expression versus ανβ3 testing in women with unexplained infertility. Fertil Steril 2014; 101: 1724-1731. [DOI:10.1016/j.fertnstert.2014.02.027]
39. Boroujerdnia MG, Nikbakht R. Beta3 integrin expression within uterine endometrium and its relationship with unexplained infertility. Pak J Biol Sci 2008; 11: 2495-2499. [DOI:10.3923/pjbs.2008.2495.2499]
40. Chen D, Jin Z, Xing F. The expression of integrin beta 3 in cycling and early pregnant endometrium and its relationship with primary unexplained infertility. Zhonghua Fu Chan Ke Za Zhi 1998; 33: 487-489.
41. Casals G, Ordi J, Creus M, Fábregues F, Carmona F, Casamitjana R, et al. Expression pattern of osteopontin and αvβ3 integrin during the implantation window in infertile patients with early stages of endometriosis. Hum Reprod 2012; 27: 805-813. [DOI:10.1093/humrep/der432]
42. Li Q, Bagchi MK, Bagchi IC. Identification of a signaling pathway involving progesterone receptor, calcitonin, and tissue tranglutaminase in Ishikawa endometrial cells. Endocrinology 2006; 147: 2147-2154. [DOI:10.1210/en.2005-1116]
43. Zhu LJ, Bagchi MK, Bagchi IC. Attenuation of calcitonin gene expression in pregnant rat uterus leads to a block in embryonic implantation. Endocrinology 1998; 139: 330-339. [DOI:10.1210/endo.139.1.5707]
44. Evron A, Goldman S, Shalev E. Effect of primary human endometrial stromal cells on epithelial cell receptivity and protein expression is dependent on menstrual cycle stage. Hum Reprod 2011; 26: 176-190. [DOI:10.1093/humrep/deq296]
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