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Volume 15, Issue 3 (5-2017)
IJRM 2017, 15(3): 141-146 |
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Mirhoseini M, Talebpour Amiri F, Karimpour Malekshah A A, Rezanejad Gatabi Z, Ghaffari E. Protective effects of melatonin on testis histology following acute torsion-detorsion in rats. IJRM 2017; 15 (3) :141-146
URL: http://ijrm.ir/article-1-809-en.html
URL: http://ijrm.ir/article-1-809-en.html
Mehri Mirhoseini1 
, Fereshteh Talebpour Amiri * 2, Abbas Ali Karimpour Malekshah3 , Zahra Rezanejad Gatabi4 , Elmira Ghaffari5
, Fereshteh Talebpour Amiri * 2, Abbas Ali Karimpour Malekshah3 , Zahra Rezanejad Gatabi4 , Elmira Ghaffari5
1- Amol Faculty of Nursing and Midwifery, Mazandaran University of Medical Sciences, Sari, Iran.
2- Department of Anatomical Sciences, Immunogenetics Research Center, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran , ftaleb2001@yahoo.co.uk
3- epartment of Anatomical Sciences, Immunogenetics Research Center, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran.
4- Department of Pharmaceutics, Faculty of Pharmacy, Mazandaran University of Medical Sciences, Sari, Iran
5- Department of Anatomical Sciences, Immunogenetics Research Center, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran.
2- Department of Anatomical Sciences, Immunogenetics Research Center, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran , ftaleb2001@yahoo.co.uk
3- epartment of Anatomical Sciences, Immunogenetics Research Center, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran.
4- Department of Pharmaceutics, Faculty of Pharmacy, Mazandaran University of Medical Sciences, Sari, Iran
5- Department of Anatomical Sciences, Immunogenetics Research Center, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran.
Abstract: (2885 Views)
Background: Testicular damage due to spermatic cord torsion may lead toinfertility. It is probably because of changes in oxidative stress factors such asmalondialdehyde.
Objective: To investigate the protective effect of melatonin (MLT), as anantioxidant, on testicular damage induced by acute unilateral spermatic cord torsionand detorsion (T/D) in rats.
Materials and Methods: In this experimental study, 48 adult male Wistar rats wererandomly divided into three groups (8 rats/group): sham group underwent rightscrotal surgery only., the T/D group underwent right testicular torsion (for 1 hr) anddetorsion, and the melatonin group underwent right testicular torsion, received 25μg/kg melatonin intraperitoneally immediately after surgery of T/D. Then thehistological parameters and malondialdehyde (MDA) changes were evaluated.
Results: Torsion and detorsion decreased the diameter of the tubules significantlycompared to controls (p=0.003). Melatonin could increase the diameter, but it wasnot significant (p=0.26). The heights of the epithelium were constant in sham, T/D,and melatonin groups without any significant difference between groups (p=0.98).Based on Johnsen’s score, spermatogenesis was normal in the sham group. Thetorsion significantly injured all lineage cells (p<0.001). There was no any spermatidor sperm in the seminiferous tubules. Melatonin improved the spermatogenesissignificantly (p=0.02), but could not improve MDA level significantly (p=0.99).
Conclusion: Severe degenerative changes of testis were induced by acute unilateralspermatic cord torsion and detorsion in rats, but it had no effect on MDA level.
Objective: To investigate the protective effect of melatonin (MLT), as anantioxidant, on testicular damage induced by acute unilateral spermatic cord torsionand detorsion (T/D) in rats.
Materials and Methods: In this experimental study, 48 adult male Wistar rats wererandomly divided into three groups (8 rats/group): sham group underwent rightscrotal surgery only., the T/D group underwent right testicular torsion (for 1 hr) anddetorsion, and the melatonin group underwent right testicular torsion, received 25μg/kg melatonin intraperitoneally immediately after surgery of T/D. Then thehistological parameters and malondialdehyde (MDA) changes were evaluated.
Results: Torsion and detorsion decreased the diameter of the tubules significantlycompared to controls (p=0.003). Melatonin could increase the diameter, but it wasnot significant (p=0.26). The heights of the epithelium were constant in sham, T/D,and melatonin groups without any significant difference between groups (p=0.98).Based on Johnsen’s score, spermatogenesis was normal in the sham group. Thetorsion significantly injured all lineage cells (p<0.001). There was no any spermatidor sperm in the seminiferous tubules. Melatonin improved the spermatogenesissignificantly (p=0.02), but could not improve MDA level significantly (p=0.99).
Conclusion: Severe degenerative changes of testis were induced by acute unilateralspermatic cord torsion and detorsion in rats, but it had no effect on MDA level.
Type of Study: Original Article |
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