International Journal of
Reproductive Biomedicine
Sun, May 19, 2024
[Archive]
Volume 16, Issue 12 (December 2018)
IJRM 2018, 16(12): 775-782 |
Back to browse issues page
Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
Hendarto H, Widyanugraha M Y A W, Widjiati W. Curcumin improves growth factors expression of bovine cumulus-oocyte complexes cultured in peritoneal fluid of women with endometriosis. IJRM 2018; 16 (12) :775-782
URL: http://ijrm.ir/article-1-1333-en.html
URL: http://ijrm.ir/article-1-1333-en.html
1- Department of Obstetrics and Gynecology, Faculty of Medicine, Airlangga University/Dr Soetomo Hospital Surabaya, Indonesia , hndhendy@yahoo.com
2- Department of Obstetrics and Gynecology, Faculty of Medicine, Airlangga University/Dr Soetomo Hospital Surabaya, Indonesia
3- Laboratory of Embriology, Faculty of Veterinary Medicine, Airlangga University, Surabaya, Indonesia.
2- Department of Obstetrics and Gynecology, Faculty of Medicine, Airlangga University/Dr Soetomo Hospital Surabaya, Indonesia
3- Laboratory of Embriology, Faculty of Veterinary Medicine, Airlangga University, Surabaya, Indonesia.
Abstract: (3972 Views)
Background: Peritoneal fluid (PF) from infertile women with endometriosis contains inflammatory mediators that may interfere with folliculogenesis.
Objective: The aim was to evaluate the effects of curcumin on growth factors expression by evaluating Growth Differentiation Factor-9 (GDF-9), Kit Ligand (KitL), and Tumor Necrosis Factor 𝛼 (TNF𝛼) expressions in bovine cumulus-oocyte complexes (COC)s cultured with PF from infertile women with endometriosis.
Materials and Methods: In this experimental study, 21 infertile women (aged between 20 and 40 years) who refered to Dr Sutomo Hospital from January to July 2015 were enrolled. COCs were aspirated from antral follicles of bovine ovaries. PF was collected from infertile women with endometriosis undergoing laparoscopy for infertility evaluation. Curcumin, a strong anti-inflammatory turmeric, was added in Tissue Culture Medium 199 (TCM199) and PF for culture medium. Bovine COCs were cultured into three groups of the medium: 1. TCM199, 2. TCM199 + PF, and 3. TCM199 + PF + curcumin. GDF-
9, KitL, and TNF𝛼 expressions were examined using immunohistochemistry technique.
Results: GDF-9 expression of bovine COCs cultured in PF with curcumin addition (2.67 ± 0.98) was found to increase compared to those cultured without curcumin (0.50 ± 0.67) (p ≤ 0.001). It was similar to KitL expression of bovine COCs cultured with curcumin (2.67 ± 1.23), which increased compared to those without curcumin (0.33 ± 0.49) (p ≤ 0.001). A significant difference in TNF𝛼 expression was noted between groups with or without curcumin (p ≤ 0.001).
Conclusion: In the culture of PF from infertile women with endometriosis, curcumin addition improves the growth factors expression of bovine COCs. The increase of GDF- 9 and KitL expressions will improve folliculogenesis.
Objective: The aim was to evaluate the effects of curcumin on growth factors expression by evaluating Growth Differentiation Factor-9 (GDF-9), Kit Ligand (KitL), and Tumor Necrosis Factor 𝛼 (TNF𝛼) expressions in bovine cumulus-oocyte complexes (COC)s cultured with PF from infertile women with endometriosis.
Materials and Methods: In this experimental study, 21 infertile women (aged between 20 and 40 years) who refered to Dr Sutomo Hospital from January to July 2015 were enrolled. COCs were aspirated from antral follicles of bovine ovaries. PF was collected from infertile women with endometriosis undergoing laparoscopy for infertility evaluation. Curcumin, a strong anti-inflammatory turmeric, was added in Tissue Culture Medium 199 (TCM199) and PF for culture medium. Bovine COCs were cultured into three groups of the medium: 1. TCM199, 2. TCM199 + PF, and 3. TCM199 + PF + curcumin. GDF-
9, KitL, and TNF𝛼 expressions were examined using immunohistochemistry technique.
Results: GDF-9 expression of bovine COCs cultured in PF with curcumin addition (2.67 ± 0.98) was found to increase compared to those cultured without curcumin (0.50 ± 0.67) (p ≤ 0.001). It was similar to KitL expression of bovine COCs cultured with curcumin (2.67 ± 1.23), which increased compared to those without curcumin (0.33 ± 0.49) (p ≤ 0.001). A significant difference in TNF𝛼 expression was noted between groups with or without curcumin (p ≤ 0.001).
Conclusion: In the culture of PF from infertile women with endometriosis, curcumin addition improves the growth factors expression of bovine COCs. The increase of GDF- 9 and KitL expressions will improve folliculogenesis.
Type of Study: Original Article |
References
1. [1] Kennedy S, Bergqvist A, Chapron C, D'Hooghe T, Dunsel- man G, Greb R, et al. ESHRE guideline for the diagnosis and treatment of endometriosis. Hum Reprod 2005; 20: 2698– -2704.
2. [2] Dunselman GA, Vermeulen N, Becker C, Calhaz-Jorge C, D'Hooghe T, De Bie B, et al. ESHRE guideline: management of women with endometriosis. Hum Reprod 2014; 29: 400– -412.
3. [3] National Institute for Health and Care Excellence. Endometriosis: diagnosis and management. NICE guideline [NG73]; 2017.
4. [4] De Graaff AA, D'Hooghe TM, Dunselman GA, Dirksen CD, Hummelshoj L, Werf EndoCost Consortium, et al. The significant effect of endometriosis on physical, mental and social wellbeing: results from an international cross- sectional survey. Hum Reprod 2013; 28: 2677–-2685. [DOI:10.1093/humrep/det284]
5. [5] Xu B, Guo N, Zhang XM, Shi W, Tong XH, Iqbal F, et al. Oocyte quality is decreased in women with minimal or mild endometriosis. Sci Rep 2015; 5: 10779. [DOI:10.1038/srep10779]
6. [6] Du YB, Gao MZ, Shi Y, Sun ZG, Wang J. Endocrine and inflammatory factors and endometriosis-associated infer- tility in assisted reproduction techniques. Arch Gynecol Obstet 2013; 287: 123–-130. [DOI:10.1007/s00404-012-2567-0]
7. [7] Hendarto H. Pathomechanism of infertility in endometrio- sis. In: Chaudhury K, Chakravarty B. Endometriosis-Basic Concepts and Current Research Trends. Rijeka, Croatia: Intech; 2012; 343–-54. [DOI:10.5772/29357]
8. [8] Malvezzi H, Da Broi MG, Meola J, Rosa E-Silva JC, Ferriani RA, Navarro PA. Peritoneal fluid of women with endometriosis reduces SOD1 in bovine oocytes in vitro maturation. Cell Tissue Res 2018; 372: 621–-628. [DOI:10.1007/s00441-018-2805-2]
9. [9] Onagbesan O, Bruggeman V, Decuypere E. Intra-ovarian growth factors regulating ovarian function in avian species: a review. Anim Reprod Sci 2009; 111: 121–-140. [DOI:10.1016/j.anireprosci.2008.09.017]
10. [10] Evron A, Blumenfeld Z, Adashi EY, Kol S. The role of growth factors in ovarian function and development. The Global Library of Women's Medicine; 2015.
11. [11] Thomas FH, Vanderhyden BC. Oocyte-granulosa cell inter- actions during mouse follicular development: regulation of kit ligand expression and its role in oocyte growth. Reprod Biol Endocrinol 2006; 4: 19. [DOI:10.1186/1477-7827-4-19]
12. [12] Senbon S, Hirao Y, Miyano T. Interactions between the oocyte and surrounding somatic cells in follicular develop- ment: lessons from in vitro culture. J Reprod Dev 2003; 49: 259–-269. [DOI:10.1262/jrd.49.259]
13. [13] Celestino JJH, Matos MHT, Saraiva MVA, Figueiredo JR. Regulation of ovarian folliculogenesis by Kit Ligand and the c-Kit system in mammals. Anim Reprod 2009; 6: 431–-439.
14. [14] Sanfins A, Rodrigues P, Albertini DF. GDF-9 and BMP-15 direct the follicle symphony. J Assist Reprod Genet 2018; 35: 1741–-1750. [DOI:10.1007/s10815-018-1268-4]
15. [15] Oral E, Olive DL, Arici A. The peritoneal environment in endometriosis. Hum Reprod Update 1996; 2: 385–-398. [DOI:10.1093/humupd/2.5.385]
16. [16] Wu MH, Hsiao KY, Tsai SJ. Endometriosis and possible inflammation markers. Gynecol Minimall Invas Ther 2015; 4: 61–-67. [DOI:10.1016/j.gmit.2015.05.001]
17. [17] Izumi G, Koga K, Takamura M, Makabe T, Satake E, Takeuchi A, et al. Involvement of immune cells in the pathogenesis of endometriosis. J Obstet Gynaecol Res 2018; 44: 191–-198. [DOI:10.1111/jog.13559]
18. [18] Jurenka JS. Anti-inflammatory properties of curcumin, a major constituent of Curcuma longa: a review of preclinical and clinical research. Altern Med Rev 2009; 14: 141-–153.
19. [19] He Y, Yue Y, Zheng X, Zhang K, Chen S, Du Z. Curcumin, inflammation, and chronic diseases: how are they linked? Molecules 2015; 20: 9183–-9213. [DOI:10.3390/molecules20059183]
20. [20] Arablou T, Kolahdouz-Mohammadi R. Curcumin and endometriosis: Review on potential roles and molecular mechanisms. Biomed Pharmacother 2018; 97: 91–-97. [DOI:10.1016/j.biopha.2017.10.119]
21. [21] Kizilay G, Uz YH, Seren G, Ulucam E, Yilmaz A, Cukur Z, et al. In vivo effects of curcumin and deferoxamine in experimental endometriosis. Adv Clin Exp Med 2017; 26: 207-–213.
22. [22] American society for reproductive medicine. Revised amer- ican society for reproductive medicine classification of endometriosis: 1996. Fertil Steril 1997; 67: 817–-821. [DOI:10.1016/S0015-0282(97)81391-X]
23. [23] Jorgensen H, Hill AS, Beste MT, Kumar MP, Chiswick E, Fedorcsak P, et al. Peritoneal fluid cytokines related to endometriosis in patients evaluated for infertility. Fertil Steril 2017; 107: 1191–-1199. [DOI:10.1016/j.fertnstert.2017.03.013]
24. [24] Nowak M, Madej JA, Dziegiel P. Intensity of COX2 expres- sion in cells of soft tissue fibrosacrcomas in dogs as related to grade of tumour malignancy. Bull Vet Inst Pulawy 2007; 51: 275–-279.
25. [25] Ardianta W MY, Hendarto H, Widjiati W. Kurkumin Menu- runkan Ekspresi Tumor Necrosis Factor (TNF)- Kompleks Oosit-Kumulus Sapi pada Kultur dengan Zalir Peritoneum Penderita Infertil Terkait Endometriosis. Majalah Obstet Ginekol 2015; 23: 133–-139. [DOI:10.20473/mog.v23i3.2079]
26. [26] Scholl B, Bersinger NA, Kuhn A, Mueller MD. Correlation between symptoms of pain and peritoneal fluid inflam- matory cytokine concentrations in endometriosis. Gynecol Endocrinol 2009; 25: 701–-706. [DOI:10.3109/09513590903159680]
27. [27] Hendarto H, Prabowo P, Moeloek FA, Soetjipto S. Growth differentiation factor 9 concentration in the follicular fluid of infertile women with endometriosis. Fertil Steril 2010; 94: 758–-760. [DOI:10.1016/j.fertnstert.2009.10.011]
28. [28] Dong J, Albertini DF, Nishimori K, Kumar TR, Lu N, Matzuk MM. Growth differentiation factor-9 is required during early ovarian folliculogenesis. Nature 1996; 383: 531–-535. [DOI:10.1038/383531a0]
29. [29] Orisaka M, Orisaka S, Jiang JY, Craig J, Wang Y, Kotsuji F, et al. Growth differentiation factor 9 is antiapoptotic during follicular development from preantral to early antral stage. Mol Endocrinol 2006; 20: 2456–-2468. [DOI:10.1210/me.2005-0357]
30. [30] Bayne RA, Kinnell HL, Coutts SM, He J, Childs AJ, Anderson RA. GDF9 is transiently expressed in oocytes before follicle formation in the human fetal ovary and is regulated by a novel NOBOX transcript. PloS One 2015; 10: e0119819. [DOI:10.1371/journal.pone.0119819]
31. [31] Carlsson IB, Laitinen MP, Scott JE, Louhio H, Velentzis L, Tuuri T, et al. Kit ligand and c-Kit are expressed during early human ovarian follicular development and their interaction is required for the survival of follicles in long-term culture. Reproduction 2006; 131: 641–-649. [DOI:10.1530/rep.1.00868]
32. [32] Mahajan A, Kumar P, Atole SA, Brahmkshtri BP, Tajane KR, Brahmkshtri BP, et al. Kit Ligand Expression in granulosa cells during follicular culture and associated maturation rate of oocytes in buffalo (Bubalus bubalis). Indian J Anim Res 2015; 50: 14–-18. [DOI:10.18805/ijar.7488]
33. [33] Gogacz M, Bogusiewicz M, Putowski L, Adamiak A, Wertel I, Jakowicki JA, et al. Expression of tumor necrosis factor- alpha (TNF-alpha) on peritoneal fluid mononuclear cells in women with endometriosis. Ginekol Pol 2008; 79: 31–-35.
34. [34] Bedaiwy MA, Falcone T, Sharma RK, Goldberg JM, Attaran M, Nelson DR, et al. Prediction of endometriosis with serum and peritoneal fluid markers: a prospective controlled trial. Hum Reprod 2002; 17: 426–-431. [DOI:10.1093/humrep/17.2.426]
35. [35] Ma CH, Yan LY, Qiao J, Sha W, Li L, Chen Y, et al. Effects of tumor necrosis factor-alpha on porcine oocyte meiosis progression, spindle organization, and chromosome align- ment. Fertil Steril 2010; 93: 920–-926. [DOI:10.1016/j.fertnstert.2009.01.131]
36. [36] Jana S, Rudra DS, Paul S, Snehasikta S. Curcumin delays endometriosis development by inhibiting MMP-2 activity. Indian J Biochem Biophys 2012; 49: 342–-348.
Send email to the article author
| Rights and permissions | |
 |
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License. |
