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Volume 19, Issue 1 (January 2021)
IJRM 2021, 19(1): 47-56 |
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Ansari A S, Sevliya K, Badar: A, Lohiya N K. Reversible inhibition of sperm under guidance as an intratubular and reversible contraception in female rats: An experimental study. IJRM 2021; 19 (1) :47-56
URL: http://ijrm.ir/article-1-1434-en.html
URL: http://ijrm.ir/article-1-1434-en.html
1- Department of Zoology, Center for Advanced Studies, University of Rajasthan, Jaipur, Rajasthan, India.
2- Department of Zoology, Center for Advanced Studies, University of Rajasthan, Jaipur, Rajasthan, India. , badrayesha@gmail.com
2- Department of Zoology, Center for Advanced Studies, University of Rajasthan, Jaipur, Rajasthan, India. , badrayesha@gmail.com
Abstract: (1393 Views)
Background: Reversible inhibition of sperm under guidance (“RISUG®”) is a promising intravasal male contraceptive.
Objective: An exploratory study was conducted with a concept of non-invasive, transcervical, single-intervention and reversible contraception using RISUG® in females.
Materials and Methods: In this experimental study, 60 adult Wistar albino female rats weighing 150-155 g, 3-4 months old were divided into four groups: group I: sham-operated control; group II: tubal occlusion with RISUG for 90 days; group III: tubal occlusion with RISUG® for 90 days and reversal with dimethyl sulphoxide and group IV: tubal occlusion with RISUG® for 90 days and reversal with 5% NaHCO3. Animals were subjected to bilateral fallopian tube occlusion with RISUG® and reversal with DMSO and NaHCO3. The estrous cycle, fertility and histology of fallopian tube were evaluated.
Results: Group I showed 100% fertility during all mating schedules. Animals of experimental groups indicated positive mating, but 0% fertility was evident following 30, 60, and 90 days of tubal occlusion. However, after reversal, fertility steadily increased to normalcy in groups III (50% at 45 days, 80% at 105 days, 100% at 150 and 195 days) and IV (70% at 45 and 105 days, 100% at 150 and 195 days) animals. Group II illustrated disorganized inner cell linings and eosinated RISUG® implant-filled lumen. Reversal groups (III and IV) revealed complete restoration of cellular histo-architecture. Regular estrous cycle was noticed in all experimental groups.
Conclusion: RISUG® is suitable for single intervention, intratubular, reversible contraception in female rats.
Objective: An exploratory study was conducted with a concept of non-invasive, transcervical, single-intervention and reversible contraception using RISUG® in females.
Materials and Methods: In this experimental study, 60 adult Wistar albino female rats weighing 150-155 g, 3-4 months old were divided into four groups: group I: sham-operated control; group II: tubal occlusion with RISUG for 90 days; group III: tubal occlusion with RISUG® for 90 days and reversal with dimethyl sulphoxide and group IV: tubal occlusion with RISUG® for 90 days and reversal with 5% NaHCO3. Animals were subjected to bilateral fallopian tube occlusion with RISUG® and reversal with DMSO and NaHCO3. The estrous cycle, fertility and histology of fallopian tube were evaluated.
Results: Group I showed 100% fertility during all mating schedules. Animals of experimental groups indicated positive mating, but 0% fertility was evident following 30, 60, and 90 days of tubal occlusion. However, after reversal, fertility steadily increased to normalcy in groups III (50% at 45 days, 80% at 105 days, 100% at 150 and 195 days) and IV (70% at 45 and 105 days, 100% at 150 and 195 days) animals. Group II illustrated disorganized inner cell linings and eosinated RISUG® implant-filled lumen. Reversal groups (III and IV) revealed complete restoration of cellular histo-architecture. Regular estrous cycle was noticed in all experimental groups.
Conclusion: RISUG® is suitable for single intervention, intratubular, reversible contraception in female rats.
Type of Study: Original Article |
Subject:
Reproductive Biology
References
1. Ceballos G, Ehrlich PR, Dirzo R. Biological annihilation via the ongoing sixth mass extinction signaled by vertebrate population losses and declines. Proc Natl Acad Sci USA 2017; 114: E6089-E6096. [DOI:10.1073/pnas.1704949114] [PMID] [PMCID]
2. Bavel JV. The world population explosion: causes, backgrounds and projections for the future. Facts Views Vis Obgyn 2013; 5: 281-291.
3. Jain R, Muralidhar S. Contraceptive methods: Needs, options and utilization. J Obstet Gynecol India 2011; 61: 626-634. [DOI:10.1007/s13224-011-0107-7] [PMID] [PMCID]
4. Jokinen E, Heino A, Karipohja T, Gissler M, Hurskainen R. Safety and effectiveness of female tubal sterilisation by hysteroscopy, laparoscopy or laparotomy: a register based study. An Int J of Obstet Gynaecol 2017; 124: 1851-1857. [DOI:10.1111/1471-0528.14719] [PMID]
5. Sharma RS, Rajanna A, Singh BK, Mathur AK, Mukherjee AK. Current Status of development of RISUG: An intravasal injectable male contraceptive. In: Sharma RS, Rajanna A, Rajalakshmi M (Eds.). Recent advances and challenges in Reproductive Health Research. New Delhi: Indian Council of Medical Research; 2008. pp: 135-149.
6. Lohiya NK, Manivannan B, Mishra PK, Pathak N, Balasubramanian SPA. Intravasal contraception with styrene maleic anhydride and its non-invasive reversal in langur monkeys (Presbytis entellus entellus). Contraception 1998; 58: 119-128. [DOI:10.1016/S0010-7824(98)00073-0]
7. Lohiya NK, Suthar R, Khandelwal A, Goyal S, Ansari AS, Manivannan B. Sperm characteristics and teratology in rats following vas occlusion with RISUG and its reversal. Int J Androl 2010; 33: e198-e206. [DOI:10.1111/j.1365-2605.2009.00992.x] [PMID]
8. Lohiya NK, Alam I, Hussain M, Khan SR, Ansari AS. RISUG: An intravasal injectable male contraceptive. Indian J Med Res 2014; 140 (Suppl.): 63-72.
9. Ansari AS, Alam I, Hussain M, Khan SR, Lohiya NK. Evaluation of genotoxicity in leukocytes and testis following intra-vasal contraception with RISUG and its reversal by DMSO and NaHCO3 in Wistar albino rats. Reprod Toxicol 2013; 36: 53-59. [DOI:10.1016/j.reprotox.2012.11.005] [PMID]
10. Ansari AS, Hussain M, Khan SR, Lohiya NK. Relative suitability of DMSO and NaHCO3 for reversal of RISUG® induced long-term contraception. Andrology 2016; 4: 306-313. [DOI:10.1111/andr.12155] [PMID]
11. Ansari AS, Badar A, Balasubramanian K, Lohiya NK. Contraception with RISUG® and functional reversal through DMSO and NaHCO3 in male rabbits. Asian J Androl 2017; 19: 389-395. [DOI:10.4103/1008-682X.185000] [PMID] [PMCID]
12. Lohiya NK, Manivannan B, Mishra PK, Sriram S, Bhande SS, Panneerdoss S. Preclinical evaluation for non-invasive reversal following long-term vas occlusion with styrene maleic anhydride in langur monkeys. Contraception 2005; 71: 214-226. [DOI:10.1016/j.contraception.2004.08.016] [PMID]
13. Chaube SK, Misro MM. Effect of styrene maleic anhydride (SMA) on viability and integrity of isolated oocytes in vitro. Contraception 2002; 66: 469-472. [DOI:10.1016/S0010-7824(02)00419-5]
14. Jha RK, Jha PK, Rana SV, Guha SK. Spermicidal action of styrene maleic anhydride polyelectrolyte in combination with magnetic and electrically conductive particle. Int J Pharmacol 2009; 5: 1-12. [DOI:10.3923/ijp.2009.1.12]
15. Guha SK. Contraceptive for use by a male. US: Patent; 54880705; 1996.
16. Goldman JM, Murr AS, Cooper RL. The rodent estrous cycle: characterization of vaginal cytology and its utility in toxicological studies. Birth Defects Research 2007; 80: 84-97. [DOI:10.1002/bdrb.20106] [PMID]
17. CPCSEA guidelines. Guidelines on the regulation of scientific experiments on animals. Available at: http://cpcsea.nic.in/WriteReadData/userfiles/file/SOP_CPCSEA_inner_page.pdf.
18. Lin ChM, Ku YL, Cheng YT, Giin NY, Ou YCh, Lee MCh, et al. An uncommon spontaneous right distal tubal pregnancy post bilateral laparoscopic sterilization. A case report. Medicine 2019; 98: e14193-e14195. [DOI:10.1097/MD.0000000000014193] [PMID] [PMCID]
19. FDA medical devices. FDA activities: Essure. page last updated 04/09/2018. Available at: https://www.fda.gov/news-events/press-announcements/fda-restricts-sale-and-distribution-essure-protect-women-and-require-patients-receive-risk.
20. Hologic. Instructions for use and radiofrequency (RF) generator operator are manual. Available at: www.adiana.com/pdf/info/adiana-patient-information-booklet.pdf.
21. Frietze G, Leyser-Whalen O, Rahman M, Rouhani M, Berenson AB. A meta-analysis of bilateral essure® procedural placement success rates on first attempt. J Gynecol Surg 2015; 31: 308-317. [DOI:10.1089/gyn.2015.0054] [PMID] [PMCID]
22. Mao J, Pfeifer S, Schlegel P, Sedrakyan A. Safety and efficacy of hysteroscopic sterilization compared with laparoscopic sterilization: an observational cohort study. Br Med J 2015; 31: 351. [DOI:10.1136/bmj.h5162] [PMID] [PMCID]
23. Clarke JJ, Sokal DC, Cancel AM, Campen DB, Gudi R, Wagner VO, et al. Re-evaluation of the mutagenic potential of quinacrine dihydrochloride dihydrate. Mutat Res 2001; 494: 41-53. [DOI:10.1016/S1383-5718(01)00178-4]
24. Lippes J. Quinacrine sterilization (QS): Time for reconsideration. Contraception 2015; 92: 91-95. [DOI:10.1016/j.contraception.2015.06.005] [PMID]
25. Jensen JT, Hanna C, Yao Sh, Micks E, Edelman A, Holden L, et al. Blockade of tubal patency following transcervical administration of polidocanol foam: initial studies in rhesus macaques. Contraception 2014; 89: 540-549. [DOI:10.1016/j.contraception.2013.11.017] [PMID] [PMCID]
26. Jensen JT, Hanna C, Yao Sh, Bauer C, Morgan TK, Slayden OD. Characterization of tubal occlusion after transcervical polidocanol foam (PF) infusion in baboons. Contraception 2015; 92: 96-102. [DOI:10.1016/j.contraception.2015.06.002] [PMID] [PMCID]
27. Yao S, Hanna C, Slayden OD, Jensen J. An ex vivo model for assessing acute effects of transcervical polidocanol foam in the macaque fallopian tube. Fertil Steril 2016; 106: e113. [DOI:10.1016/j.fertnstert.2016.07.338]
28. Briceag I, Costache A, Purcarea VL, Cergan R, Dumitru M, Briceag I, et al. Fallopian tubes: Literature review of anatomy and etiology in female infertility. J Med Life 2015; 8: 129-131.
29. Eggert-Kruse W, Kohler A, Rohr G, Runnebaum B. The pH as an important determinant of sperm mucus interaction. Fertil Steril 1993; 59: 617-628 [DOI:10.1016/S0015-0282(16)55810-5]
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