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Volume 19, Issue 1 (January 2021)
IJRM 2021, 19(1): 75-86 |
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Nikoo S, Ebtekar M, Jeddi-Tehrani M, Bozorgmehr M, Zarnani A H. Culture density of menstrual blood-derived stromal/stem cells determines the quality of T cell responses: An experimental study. IJRM 2021; 19 (1) :75-86
URL: http://ijrm.ir/article-1-1736-en.html
URL: http://ijrm.ir/article-1-1736-en.html
Shohreh Nikoo1 
, Massoumeh Ebtekar2 , Mahmood Jeddi-Tehrani3 , Mahmood Bozorgmehr4 , Amir Hassan Zarnani * 5
, Massoumeh Ebtekar2 , Mahmood Jeddi-Tehrani3 , Mahmood Bozorgmehr4 , Amir Hassan Zarnani * 5
1- Immunology Research Center, Iran University of Medical Sciences, Tehran, Iran.
2- Department of Immunology, Faculty of Medicine, Tarbiat Modares University, Tehran, Iran.
3- Monoclonal Antibody Research Center, Avicenna Research Institute, ACECR, Tehran, Iran.
4- Reproductive Immunology Research Center, Avicenna Research Institute, ACECR, Tehran, Iran. Oncopathology Research Center, Iran University of Medical Sciences, Tehran, Iran.
5- Reproductive Immunology Research Center, Avicenna Research Institute, ACECR, Tehran, Iran. Department of Immunology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran. , zarnania@gmail.com
2- Department of Immunology, Faculty of Medicine, Tarbiat Modares University, Tehran, Iran.
3- Monoclonal Antibody Research Center, Avicenna Research Institute, ACECR, Tehran, Iran.
4- Reproductive Immunology Research Center, Avicenna Research Institute, ACECR, Tehran, Iran. Oncopathology Research Center, Iran University of Medical Sciences, Tehran, Iran.
5- Reproductive Immunology Research Center, Avicenna Research Institute, ACECR, Tehran, Iran. Department of Immunology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran. , zarnania@gmail.com
Abstract: (1526 Views)
Background: Menstrual blood-derived stromal/stem cells (MenSCs) are a new population of refreshing and highly proliferative stem cells. Immunomodulatory effects of MenSCs profoundly depend on their relative density.
Objective: To find whether MenSCs cultured at varying numbers would differentially affect the allogenic peripheral blood mononuclear cells (PBMCs) key features.
Materials and Methods: PBMCs were co-cultured with various MenSCs numbers. PBMCs proliferation was investigated via 3H-thymidine incorporation. Flow cytometry was used to assess human leukocyte antigen (HLA)-DR, HLA-ABC, HLA-G, and co-stimulatory markers on MenSCs and the percentage of regulatory T cells (Tregs) among PBMCs. The concentration of cytokines was determined in supernatant of co-cultures.
Results: The support of PBMCs proliferation at low MenSCs densities correlated with higher levels of pro-inflammatory interferon gamma (IFN-γ) in MenSCs/PBMCs co-culture and increased expression of HLA-DR by MenSCs. On the other hand, the suppressive property of MenSCs at higher densities was independent of Treg frequency, but correlated with a high concentration of Interleukin (IL)-6 and IL-10 in the co-cultures.
Conclusion: Totally, at different seeding densities, MenSCs could differentially interact with PBMCs leading to significant changes in the level of anti- and/or pro-inflammatory factors. These preliminary in vitro results are suggested to be taken into consideration in experimental models of MenSC-based immunomodulation. Nonetheless, for efficient utilization of MenSCs anti-inflammatory features in pre-clinical disease models, we still need to broaden our knowledge on MenSC-immune system cross-talk; this could play a part in designing more optimized MenSCs injection modalities in the case of future pre-clinical and subsequently clinical settings.
Objective: To find whether MenSCs cultured at varying numbers would differentially affect the allogenic peripheral blood mononuclear cells (PBMCs) key features.
Materials and Methods: PBMCs were co-cultured with various MenSCs numbers. PBMCs proliferation was investigated via 3H-thymidine incorporation. Flow cytometry was used to assess human leukocyte antigen (HLA)-DR, HLA-ABC, HLA-G, and co-stimulatory markers on MenSCs and the percentage of regulatory T cells (Tregs) among PBMCs. The concentration of cytokines was determined in supernatant of co-cultures.
Results: The support of PBMCs proliferation at low MenSCs densities correlated with higher levels of pro-inflammatory interferon gamma (IFN-γ) in MenSCs/PBMCs co-culture and increased expression of HLA-DR by MenSCs. On the other hand, the suppressive property of MenSCs at higher densities was independent of Treg frequency, but correlated with a high concentration of Interleukin (IL)-6 and IL-10 in the co-cultures.
Conclusion: Totally, at different seeding densities, MenSCs could differentially interact with PBMCs leading to significant changes in the level of anti- and/or pro-inflammatory factors. These preliminary in vitro results are suggested to be taken into consideration in experimental models of MenSC-based immunomodulation. Nonetheless, for efficient utilization of MenSCs anti-inflammatory features in pre-clinical disease models, we still need to broaden our knowledge on MenSC-immune system cross-talk; this could play a part in designing more optimized MenSCs injection modalities in the case of future pre-clinical and subsequently clinical settings.
Type of Study: Original Article |
Subject:
Stem Cell & Cloning
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