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کاهش برازندگی شب‌پره مینوز گوجه‌فرنگی با القای تغییرات سیستمیک در شاخسار هورمون پاشی‌شده گیاه ناشی از برهم‌کنش‌های رقم و هورمون

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانش‌آموخته - دانشگاه بوعلی‌سینا

2 هیات علمی - دانشگاه بوعلی‌سینا

چکیده

هورمون‌های گیاهی از ترکیباتی هستند که به دنبال حمله گیاه‌خواران افزایش یافته و توانایی دفاعی گیاه را بالا می‌برند. ایده کاربرد خارجی هورمون روی محصولات گیاهی به منظور القای مقاومت نسبت به حشرات گیاه‌خوار با بررسی واکنش‌های رفتاری و فیزیولوژیک شب‌پره مینوز گوجه‌فرنگی، Tuta absoluta (Lepidoptera: Gelechiidae) روی سه رقم گوجه‌فرنگی شامل دهقان، فلات-111 و موبیل در پاسخ به تیمارهای هورمونی اسید جاسمونیک و اسید سالیسیلیک با اندازه‌گیری ترجیح تخم‌گذاری حشرات ماده، زنده‌مانی و مدت نمو هر یک از مراحل نارس و مقدار کربوهیدرات‌ها و فنل‌های محلول در برگ‌ها مورد مطالعه قرار گرفت. ترجیح تخم‌گذاری افراد ماده و مدت تکوین جنین روی رقم حساس فلات-111 به طور قابل ملاحظه‌ای در واحد‌های آزمایشی اسید جاسمونیک به ترتیب کاهش و افزایش یافتند. نرخ نمو لارو بیشترین واکنش را نسبت به تیمارهای هورمونی در رقم نیمه مقاوم دهقان از خود نشان داد. زنده‌مانی و نرخ نمو شفیره‌ها نیز به طور قابل توجهی تحت تاثیر تیمارهای اسید جاسمونیک روی هر سه رقم کاهش یافت. با وجود (علی‌رغم) تفاوت‌های معنی‌دار در غلظت کربوهیدرات‌ها و فنل‌های محلول برگ ارقام گوجه‌فرنگی بین واحد‌های آزمایشی شاهد و هورمون‌پاشی‌شده و همچنین با وجود همبستگی مثبت معنی‌دار بین تغییرات غلظت کربوهیدرات‌ها و فنل‌ها در واحد‌های آزمایشی، همبستگی معناداری بین محتویات گیاهی اندازه‌گیری‌شده و متغیر‌های زیستی شب‌پره مینوز گوجه‌فرنگی مشاهده نشد. نتایج نشان داد که هورمون‌های مذکور در تلفیق با ارقام مناسب گیاهی می‌توانند برای ایجاد تغییرات رفتاری و فیزیولوژیک در این آفت مورد استفاده قرار گیرند.

کلیدواژه‌ها


عنوان مقاله [English]

Systemic changes in tomato induced by foliar treated hormone and cultivar interactions reduce the fitness of an invasive specialist herbivore, tomato leafminer

نویسندگان [English]

  • Azim Nemati 1
  • Babak Zahiri 2
  • Mohammad Khanjani 2
1 MSc Graduated
2 Academic Staff - Bu-Ali Sina University
چکیده [English]

Plant hormones are the compounds that are increased followed by the attack of herbivores to enhance plant defense ability. The idea of external application of hormones on crop plants to induce resistance to herbivores has been studied by examining the behavioral and physiological responses of tomato leafminer, Tuta absoluta (Lepidoptera: Gelechiidae) on three tomato cultivars including Dehghan, Falat-111 and Mobil in response to jasmonic acid and salicylic acid hormone treatments by measuring the oviposition preference of the females, survivorship and development time of each immature stage and the concentration of soluble carbohydrates and phenols in the leaves. Oviposition preference of females and embryo development time on the susceptible cultivar, Falat-111, were significantly reduced and increased respectively in jasmonic acid treatment units. Larval development rate showed the highest response to hormonal treatments in the semi-resistant cultivar, Dehghan. Survivorship and development rates of the pupae were also significantly reduced under the influence of jasmonic acid treatments on all three cultivars. Despite significant differences in leaf soluble carbohydrates and phenols concentrations between control and sprayed tomato cultivars as well as a significant positive correlation between changes in carbohydrates and phenols concentrations in the experimental units, no significant correlation was observed between the aforementioned plant contents and insect biological variables. The results showed that these hormones in combination with appropriate plant varieties can be used to induce behavioral and physiological changes in the pest.

کلیدواژه‌ها [English]

  • induced resistance
  • jasmonic acid
  • oviposition preference
  • salicylic acid
  • survivorship and development time
  1. Agrawal, A. A. & Kurashige, N. S. (2003). A role for isothiocyanates in plant resistance against the specialist herbivore Pieris rapae. Journal of Chemical Ecology, 29(6), 1403-1415.
  2. Beauverie, J. (1901). Essais d’immunisation des végétaux contre les maladies cryptogamiques. Comptes Rendus de l Académie des Sciences - Series III, 133, 107-110.
  3. Bernays, E. A. & Chapman, R. F. (2007). Host-plant selection by phytophagous insects (Vol. 2). Springer Science & Business Media.
  4. Bruinsma, M., Van Dam, N. M., Van Loon, J. J. & Dicke, M. (2007). Jasmonic acid-induced changes in Brassica oleracea affect oviposition preference of two specialist herbivores. Journal of Chemical Ecology, 33(4), 655-668.
  5. Çetin, H., Arslan, D. & Musa Özcan, M. (2011). Influence of Eriophyid mites (Aculus olearius Castagnoli and Aceria oleae (Nalepa)(Acarina: Eriophyidae)) on some physical and chemical characteristics of Ayvalık variety olive fruit. Journal of the Science of Food and Agriculture, 91(3), 498-504.
  6. Cipollini, D. F. & Redman, A. M. (1999). Age-dependent effects of jasmonic acid treatment and wind exposure on foliar oxidase activity and insect resistance in tomato. Journal of Chemical Ecology, 25(2), 271-281.
  7. Coruh, S. & Ercisli, S. (2010). Interactions between galling insects and plant total phenolic contents in Rosa canina L. genotypes. Scientific Research and Essays, 5(14), 1935-1937.
  8. Davies, P. (1995). Plant Hormones: Physiology, Biochemistry and Molecular Biology (No. 581.1/D249). Kluwer Academic Publishers.
  9. Dent, D. (2000). Host plant resistance. In: D. Dent (Ed), Insect Pest Management. (pp. 123-179.) CABI.
  10. Edreva, A. (2004). A novel strategy for plant protection: Induced resistance. Journal of Cell and Molecular Biology, 3(2), 61-69.
  11. Endo, N., Hirakawa, I., Wada, T. & Tojo, S. (2007). Induced resistance to the common cutworm, Spodoptera litura (Lepidoptera: Noctuidae) in three soybean cultivars. Applied Entomology and Zoology, 42(2), 199-204.
  12. Fischer, C., & Höll, W. (1991). Food reserves of Scots pine (Pinus sylvestris L.). Trees, 5(4), 187-195.
  13. Fouad, H. A., El-Gepaly, H. M. K. H. & Fouad, O. A. (2016). Nanosilica and jasmonic acid as alternative methods for control Tuta absoluta (Meyrick) in tomato crop under field conditions. Archives of Phytopathology and Plant Protection, 49(13-14), 362-370.
  14. Hammerschmidt, R., Nicholson, R. L. (1999). A survey of plant defense responses to pathogens. In: A. A. Agrawal, S. Tuzun. & E. Bent (Eds), Induced Plant Defenses Against Pathogens and Herbivores. (pp. 55-71). APS Press, St. Paul, Minnesota.
  15. Harpreet, K., Poonam, S. & Geetika, S. (2013). Sugar accumulation and its regulation by jasmonic acid in Brassica napus L. under salt stress. Journal of Stress Physiology & Biochemistry, 9(4).
  16. Huang, X., Renwick, J. A. A., & Sachdev-Gupta, K. (1993). Oviposition stimulants and deterrents regulating differential acceptance of Iberis amara by Pieris rapae and P. napi oleracea. Journal of Chemical Ecology, 19(8), 1645-1663.
  17. Irannejad-Parizi, L., Zahiri, B., Babolhavaeji, H., Khanjani, M. & Shararbar, H. (2015). Evaluation of twelve tomato cultivars for resistance to tomato leafminer, Tuta absoluta (Lep.: Gelechiidae). Plant Pest Research, 5, 49-61. (In Farsi with English summary)
  18. Irigoyen, J. J., Einerich, D. W. & Sánchez‐Díaz, M. (1992). Water stress induced changes in concentrations of proline and total soluble sugars in nodulated alfalfa (Medicago sativa) plants. Physiologia Plantarum, 84(1), 55-60.
  19. Kochi, S. C. & Kaliwal, B. B. (2005). Effect of Salicylic Acid on Commercial Traits of the Bivoltine Crossbreed Races of the Silkworm, Bombyx mori L. Caspian Journal of Environmental Sciences, 3(2), 107-115.
  20. Kranthi, S. (2002). Wound inducible defense related proteins in cotton against Helicoverpa armigera. Indian Journal of Entomology, 64, 73-79.
  21. Lu, Y. B., Liu, S. S., Liu, Y. Q., Furlong, M. J. & Zalucki, M. P. (2004). Contrary effects of jasmonate treatment of two closely related plant species on attraction of and oviposition by a specialist herbivore. Ecology Letters, 7(4), 337-345.
  22. Maleck, K., Levine, A., Eulgem, T., Morgan, A., Schmid, J., Lawton, K. A. & Dietrich, R. A. (2000). The transcriptome of Arabidopsis thaliana during systemic acquired resistance. Nature Genetics, 26(4), 403-410.
  23. Martinez-Abarca, F., Herrera-Cervera, J. A., Bueno, P., Sanjuan, J., Bisseling, T. & Olivares, J. (1998). Involvement of salicylic acid in the establishment of the Rhizobium meliloti-alfalfa symbiosis. Molecular Plant-Microbe Interactions, 11(2), 153-155.
  24. Panda, N. & Khush, G. S. Host plant resistance to insects. 1995. CAB International. Wallingford, United Kingdom.
  25. Ray, J. (1901). Les maladies cryptogamiques des végétaux. Revue Générale de Botanique, 13, 145-151.
  26. Renwick, J. A. A. & Chew, F. S. (1994). Oviposition behavior in Lepidoptera. Annual Review of Entomology, 39(1), 377-400.
  27. Rudell, D. R., Mattheis, J. P., Fan, X. & Fellman, J. K. (2002). Methyl Jasmonate Enhances Anthocyanin Accumulation and Modifies Production of Phenolics and Pigments in Fuji' Apples. Journal of the American Society for Horticultural Science, 127(3), 435-441.
  28. Saikia, R., Singh, T., Kumar, R., Srivastava, J., Srivastava, A. K., Singh, K. & Arora, D. K. (2003). Role of salicylic acid in systemic resistance induced by Pseudomonas fluorescens against Fusarium oxysporum f. sp. ciceri in chickpea. Microbiological Research, 158(3), 203-213.
  29. Sarfraz, M., Dosdall, L. M. & Keddie, B. A. (2006). Diamondback moth–host plant interactions: implications for pest management. Crop protection, 25(7), 625-639.
  30. SAS Institute Inc. 2013. Base SAS® 9.4 Procedures Guide. Cary, NC: SAS Institute Inc.
  31. Schenk, P. M., Kazan, K., Wilson, I., Anderson, J. P., Richmond, T., Somerville, S. C. & Manners, J. M. (2000). Coordinated plant defense responses in Arabidopsis revealed by microarray analysis. Proceedings of the National Academy of Sciences, 97(21), 11655-11660.
  32. Senthil-Nathan, S., Kalaivani, K., Choi, M. Y. & Paik, C. H. (2009). Effects of jasmonic acid-induced resistance in rice on the plant brownhopper, Nilaparvata lugens Stål (Homoptera: Delphacidae). Pesticide Biochemistry and Physiology, 95(2), 77-84.
  33. Singh, A. K. (1997). Effect of leguminous plants on the growth and development of gram pod borer, Helicoverpa armigera. Indian Journal of Entomology, 59, 209-214.
  34. Singleton, V. L. & Rossi, J. A. (1965). Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents. American journal of Enology and Viticulture, 16(3), 144-158.
  35. Smith, C. M. (2005). Plant resistance to arthropods: molecular and conventional approaches. Springer Science & Business Media.
  36. Soldaat, L. L., Boutin, J. P. & Derridj, S. (1996). Species-specific composition of free amino acids on the leaf surface of four Senecio species. Journal of Chemical Ecology, 22(1), 1-12.
  37. Stout, M. J., Workman, K. V., Bostock, R. M., & Duffey, S. S. (1998). Stimulation and attenuation of induced resistance by elicitors and inhibitors of chemical induction in tomato (Lycopersicon esculentum) foliage. Entomologia Experimentalis et Applicata, 86(3), 267-279.
  38. Strapasson, P., Pinto-Zevallos, D. M., Paudel, S., Rajotte, E. G., Felton, G. W. & Zarbin, P. H. (2014). Enhancing plant resistance at the seed stage: low concentrations of methyl jasmonate reduce the performance of the leaf miner Tuta absoluta but do not alter the behavior of its predator Chrysoperla externa. Journal of Chemical Ecology, 40(10), 1090-1098.
  39. Thaler, J. S. (1999). Jasmonate-inducible plant defences cause increased parasitism of herbivores. Nature, 399(6737), 686-688.
  40. Thaler, J. S., Stout, M. J., Karban, R. & Duffey, S. S. (1996). Exogenous jasmonates simulate insect wounding in tomato plants (Lycopersicon esculentum) in the laboratory and field. Journal of Chemical Ecology, 22(10), 1767-1781.
  41. van Lenteren, J. C. & Noldus, L. P. J. J. (1990). Whitefly-plant relationships: behavioural and ecological aspects. In: D. Gerling (Ed), Whiteflies: their bionomics, pest status and management. (pp. 47-49). Intercept Ltd, Andover, Hants, UK.
  42. van Wees, S. C., Chang, H. S., Zhu, T. & Glazebrook, J. (2003). Characterization of the early response of Arabidopsis to Alternaria brassicicola infection using expression profiling. Plant Physiology, 132(2), 606-617.
  43. War, A. R., Paulraj, M. G., Ahmad, T., Buhroo, A. A., Hussain, B., Ignacimuthu, S. & Sharma, H. C. (2012). Mechanisms of plant defense against insect herbivores. Plant Signaling & Behavior, 7(10), 1306-1320.
  44. War, A. R., Paulraj, M. G., War, M. Y. & Ignacimuthu, S. (2011). Jasmonic acid-mediated-induced resistance in groundnut (Arachis hypogaea L.) against Helicoverpa armigera (Hubner)(Lepidoptera: Noctuidae). Journal of Plant Growth Regulation, 30(4), 512-523.
  45. Yan, Z., Yan, Y. & Wang, C. (2005). Attractiveness of tobacco volatiles induced by Helicoverpa armigera and Helicoverpa assulta to Campoletis chlorideae. Chinese Science Bulletin, 50(13), 1334-1341.