Document Type : Research Paper

Authors

• mohammad zabet ¹
• hamed naseri ²
• zohreh alizadeh ³

¹ university of birjand

² Department of Agronomy and Plant Breeding, Faculty of Agriculture, university of Birjand

³ Assistant Professor in Department of Agronomy and Plant Breeding, Faculty of Agriculture, university of Birjand

Abstract

Understanding the genetic diversity and the relationships between species and plant ecotypes is an important step to preserve genetic resources. In this study, the genetic diversity of 15 Guava (Psidium guajava) genotypes was investigated using Rapid and ISSR markers in the molecular breeding laboratory of the Faculty of Agriculture, University of Birjand. Nine out of 36 RAPD primers and six out of seven ISSR primers showed polymorphism. In total, RAPD and ISSR primers amplified 80 and 35 bands, respectively, which 54 out of 80 (67.5%) and 25 out of 35 (71.42%) bands were amplified by RAPD and USSR primers, were polymorphic, respectively. The lowest and highest polymorphic information content was observed in RAPD marker in OPAB09 primer (0.13) and OPAB17 primer (0.40) and in ISSR primer in ISSR3 primer (0.19) and ISSR5 primer (0.37), respectively. According to the RAPD marker, genotypes M5 with M4 (0.83) had the highest and genotypes CH5 with R4 (0.19) had the least similarity, respectively. According to ISSR marker, genotypes R2 with R3 (0.86) had the highest and M1 with CH4 (0.12) had the least similarity, respectively. The Cluster analysis classified genotypes according to RAPD marker into five groups and according to ISSR marker into three groups, respectively. In total, the results showed that there is sufficient genetic diversity among Guava genotypes and RAPD and ISSR are useful markers in identifying polymorphic regions and estimating genetic distance and managing genetic resources of Guava.

Keywords

• Cluster analysis
• Heterozygosity
• Molecular Index
• Similarity matrix

Main Subjects

• Cellular biology